Research Articles
Beyond IC50: The 50-BOA Method for Accurate Ki Determination from Single-Point Inhibition Data
This article presents a comprehensive guide to the 50-BOA (Binding at 50% Occupancy and Activity) method, a transformative approach for estimating enzyme-inhibitor dissociation constants (Ki) using data from a single...
Mastering the 4-Parameter Logistic Model: A Complete Guide to Accurate IC50 Calculation in Drug Discovery
This comprehensive guide provides researchers, scientists, and drug development professionals with a complete framework for understanding, applying, and validating the 4-Parameter Logistic (4PL) model for IC50 determination.
Beyond the Fold: How 3D Templates Revolutionize Enzyme Functional Site Prediction in Drug Discovery
This article provides a comprehensive guide to 3D template-based methods for predicting enzyme functional sites, crucial for structure-based drug design.
The 2-His-1-Carboxylate Facial Triad: Catalytic Mechanism, Enzyme Design, and Therapeutic Targeting
This comprehensive review examines the 2-His-1-carboxylate facial triad, a conserved structural motif central to the catalytic activity of mononuclear non-heme iron(II) oxygenases.
Enzyme Kinetic Parameter Identifiability Analysis: Bridging Theory, Experiment, and Computation for Reliable Models
This article provides a comprehensive analysis of the identifiability of enzyme kinetic parameters—a fundamental challenge in creating reliable biochemical models for research, drug development, and biocatalysis.
A Comprehensive Guide to Benchmarking Monte Carlo Methods for Reliable Parameter Estimation in Biomedical Research
This article provides a systematic guide for researchers and drug development professionals on benchmarking Monte Carlo methods for parameter estimation.
From Basics to Bench: A Statistical Guide to Comparing Enzyme Kinetic Estimation Methods
This article provides a comprehensive guide for researchers and drug development professionals on the statistical comparison of enzyme kinetic estimation methods.
Validating Enzyme Kinetic Parameters: A Practical Guide to the STRENDA Guidelines for Reproducible Research
This article provides a comprehensive guide for researchers, scientists, and drug development professionals on the validation of enzyme kinetic parameters using the STRENDA (Standards for Reporting Enzymology Data) Guidelines.
Assessing the Reliability of Reported Enzyme Kinetic Parameters: A Framework for Accurate Research and Drug Development
This article provides a comprehensive guide for researchers, scientists, and drug development professionals on evaluating the reliability of reported enzyme kinetic parameters (e.g., Km, kcat, Vmax).
Beyond Linearity: A Comprehensive Guide to Traditional and Nonlinear Methods in Modern Drug Development
This article provides a definitive comparison of traditional linearization and modern nonlinear methods tailored for biomedical research and drug development.