Research Articles
The Ultimate Guide to P450-Glo Assays: High-Throughput Cytochrome P450 Screening for Drug Discovery
This comprehensive guide provides researchers and drug development professionals with an in-depth exploration of Cytochrome P450-Glo™ luciferase reporter assays for high-throughput screening (HTS).
Decoding Enzyme Mutations: A Modern Guide to Michaelis-Menten Kinetics for Drug Discovery
This comprehensive guide provides researchers and drug development professionals with a contemporary framework for applying Michaelis-Menten kinetics to characterize mutant enzymes.
Mastering the Michaelis-Menten Equation: A Comprehensive Guide to Derivation, Assumptions, and Modern Applications in Drug Discovery
This article provides a detailed, expert-level analysis of the Michaelis-Menten equation, tailored for researchers, scientists, and drug development professionals.
Mastering the MTT Assay: A Step-by-Step Guide to Accurate IC50 Determination for Drug Discovery
This comprehensive guide provides researchers and drug development professionals with a complete protocol for performing the MTT assay to determine the half-maximal inhibitory concentration (IC50) of compounds.
Advanced LAMP Assays: Harnessing Strand-Displacing Polymerases for Rapid, High-Sensitivity Molecular Diagnostics
This comprehensive guide explores the critical role of strand-displacing DNA polymerases in Loop-Mediated Isothermal Amplification (LAMP) assays, a cornerstone technology for point-of-care and field-deployable diagnostics.
IUBMB Enzyme Nomenclature Committee Guidelines: A Researcher's Guide to Classification, Nomenclature, and Modern Applications
This article provides a comprehensive guide to the International Union of Biochemistry and Molecular Biology (IUBMB) Enzyme Nomenclature Committee (NC-IUBMB) recommendations for researchers and drug development professionals.
Precise Inhibition Constant Determination: A Comprehensive Guide to ITC Calorimetry Methods for Drug Discovery
Isothermal Titration Calorimetry (ITC) has emerged as a powerful, label-free technique for quantifying biomolecular interactions, including the critical determination of inhibition constants (Ki).
Beyond the Static Value: Why Kinetics Are Essential for Accurate IC50 Determination in Drug Discovery
This article provides a comprehensive guide for researchers and drug development professionals on integrating kinetic parameters into IC50 value confirmation.
Navigating Unstable Controls in IC50 Assays: A Comprehensive Guide for Accurate Drug Potency Estimation
This article addresses the critical challenge of unstable positive and negative controls in IC50 estimation assays, a common issue that can compromise data reliability in drug discovery and development.
The Haldane Model Explained: Decoding Substrate Inhibition in Enzyme Kinetics and Drug Discovery
This article provides a comprehensive analysis of the Haldane model for substrate inhibition, a critical concept in enzymology and drug development.