Research Articles
Continuous vs. Stopped Assay Methods: A Strategic Guide to Kinetic Parameter Estimation for Drug Discovery
This article provides a comprehensive, practical framework for researchers and drug development professionals to select and optimize enzyme activity assay formats for kinetic parameter estimation.
Strategies for Robust Parameter Estimation from Incomplete Experimental Datasets in Biomedical Research
Parameter estimation from partial or incomplete experimental data is a pervasive challenge in biomedical and clinical research, impacting the reliability of models in pharmacokinetics, systems biology, and drug development.
Beyond Trial and Error: Leveraging the Fisher Information Matrix for Precision Enzyme Experimental Design
This article provides a comprehensive guide for researchers and drug development professionals on applying the Fisher Information Matrix (FIM) to optimize enzyme kinetic experiments.
Kron Reduction Method for Parameter Estimation: Solving Ill-Posed Problems in Biochemical Kinetic Modeling
This article provides a comprehensive guide to the Kron reduction method for parameter estimation in kinetic models of chemical reaction networks (CRNs), specifically addressing the common challenge of incomplete experimental...
Multi-Objective Particle Swarm Optimization in Enzyme Kinetics: Advancing Drug Discovery and Bioprocess Design
Optimizing enzymatic systems, critical for drug discovery and bioprocess engineering, involves navigating complex, high-dimensional parameter spaces with competing objectives.
Mastering Kinetic Parameter Estimation: A Comprehensive Guide to Global Optimization Methods for Biomedical Research
Accurate kinetic parameters are fundamental to constructing predictive models of biochemical systems, drug-target interactions, and cellular signaling pathways.
Mastering ReKinSim: A Comprehensive Tutorial for Reaction Kinetics Simulation in ADC and Drug Development
This comprehensive tutorial provides researchers, scientists, and drug development professionals with a complete guide to using the ReKinSim reaction kinetics simulator.
Mastering Michaelis-Menten Kinetics with NONMEM: A Comprehensive Guide to Parameter Estimation for Drug Development
This article provides a comprehensive guide for researchers and drug development professionals on estimating Michaelis-Menten pharmacokinetic parameters using NONMEM, the industry-standard nonlinear mixed-effects modeling software [citation:7].
Mastering Nonlinear Least Squares for Accurate Enzyme Kinetic Parameter Estimation in Drug Development
This article provides a comprehensive guide to nonlinear least squares (NLLS) parameter estimation for enzyme kinetics, tailored for researchers and drug development professionals.
Harnessing Uncertainty: A Practical Guide to Bayesian Parameter Estimation in Enzyme Kinetics
This article provides a comprehensive guide to Bayesian parameter estimation in enzyme kinetics, tailored for researchers, scientists, and drug development professionals.